thr77 login Options
thr77 login Options
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in many tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was found to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a significant purpose in marketing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are explained. Two extra splice variants had been reported but in-depth nucleotide sequence info just isn't nevertheless available.
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Blocking peptides are peptides that bind especially towards the goal antibody and block antibody binding. These peptide commonly consists of the epitope regarded because of the antibody.
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This guide outlines further possible optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This information outlines additional feasible optimization of cellular and immunoassay parameters to make certain the very best results are...
Antibodies certain to the blocking peptide now not bind for the epitope over the focus on protein. By evaluating the staining in the blocked antibody vs . the antibody by itself, one can see which staining is distinct.
Goal Details Cyclin E belongs to your highly conserved cyclin loved ones, whose customers are characterized by a dramatic periodicity in protein abundance from the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins show distinctive expression and degradation patterns which lead towards the temporal coordination of each and every mitotic function. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates at the G1-S period boundary and is degraded as cells development as a result of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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